Publication Date
2009
Document Type
Thesis
Committee Members
David Goldstein (Advisor), Lynn Hartzler (Committee Member), Barbara Hull (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Aquaporin 1 is a cell membrane integral protein, which functions for water transport through the hydrophobic cell membrane to the cytoplasm. HC-1 is an aquaporin 1-like protein expressed in Hyla chrysoscelis and amphibian that survives at sub-freezing temperatures in its natural habitat. The goal of the current study was to investigate the expression of HC-1 during changing physiological conditions. The expression level of HC-1 was determined in skin, muscle, liver, kidney, and intestines of warm, cold, frozen, and dehydrated frogs using Western blot and immunohistofluorescence. Although HC-1 was widely distributed, the expression level varied among tissues and physiological conditions. In addition, HC-1 was present in both glycosylated and deglycosylated forms, the pattern of glycosylation also varied with tissues and physiological conditions. Thus, I conclude that HC-1 is widely expressed in tissues of Hyla chrysoscelis, and that it is regulated in these tissues according to physiological demands.
Page Count
83
Department or Program
Department of Biological Sciences
Year Degree Awarded
2009
Copyright
Copyright 2009, all rights reserved. This open access ETD is published by Wright State University and OhioLINK.
