Ameliorations of Olea Ferruginea Fruit Pulp Extract in CCl4 induced Testicular Pathology in Mice

Authors

Syeda Nadia Ahmad, Department of Zoology, University of Chakwal, Chakwal, PakistanFollow
Tasleem Ishaq, Department of Zoology, University of Sargodha, Sargodha, PakistanFollow
Muhammad Ali Kanwal, Department of Zoology, University of Sargodha, Sargodha, PakistanFollow
Iram Inayat, Department of Zoology, University of Sargodha, Sargodha, PakistanFollow
Usma Abdullah, Department of Zoology, University of Sargodha, Sargodha, PakistanFollow
Khawaja Raees Ahmad, Department of Zoology, University of Sargodha, Sargodha, PakistanFollow

Abstract

In present study the testicular histo-ameliorations of wild olive (Olea ferruginea) fruit pulp extract (OFPE) against intra-gastric CCl₄ exposure in mice was investigated. Thirty male albino mice were allocated into three groups of ten each: 1) Vehicle control (Vc); 2) CCl₄ (C); and 3) CCl₄+ Olea ferruginea (COf). All groups, except the Vc, were administered CCl₄ (0.1mL of a 0.2mLkg⁻¹ solution) in corn oil. Over the subsequent five days, the COf group received 0.1mL of Olea ferruginea plant extract (OFPE) daily, while the Vc and CCl₄ groups were provided water in place of OFPE. Testes were recovered at day 7. Within the CCl4 group, there was significant misalignment of spermatogenic cells, such as spermatogonia, spermatocytes, spermatids, and tailless sperm heads, observed in the seminiferous tubules, along with a concomitant depletion of interstitial tissue. Partial recovery of the interstitial and spermatogonial density and realignment of spermatogenic cells were seen in COf group. Nevertheless, aggregates of dislocated spermatogenic cells were present in the middle of the seminiferous tubules. Micrometric analysis showed significant decrease (P < 0.05) in cross-sectional area (CSA) of the seminiferous tubules in COf than the Vc and Cgroups. Contrary to the sperm tail length the CSA’s of spermatogenic cell were significantly higher in C than the Vc and COf groups. So results show that OFPE can rescue the ruined testicular parameters of CCl₄ exposure.

Article History

Received: Jul 09, 2024; Accepted: Sep 09, 2024; Published: Sep 30, 2024

Recommended Citation

Ahmad, S. N., Ishaq, T., Kanwal, M., Inayat, I., Abdullah, U., & Ahmad, K. (2024). Ameliorations of Olea Ferruginea Fruit Pulp Extract in CCl4 induced Testicular Pathology in Mice, Journal of Bioresource Management, 11 (3).