Tumor Necrosis Factor Alpha SNP Variant in Promoter Region G308a, cause Preeclampsia during Pregnancy in Pakistani Women, A Case Control Study

Authors

Azizah Ziauddin, Department of Zoology, University of the Punjab, LahoreFollow
Sadia Ahmad, Allama Iqbal Medical College, Jinnah Hospital, LahoreFollow
Asad Iqbal, Department of Zoology, University of the Punjab, LahoreFollow
Abdul Majid Khan, Department of Zoolgy, University of the Punjab, LahoreFollow
Shahid Mahmood Baig, National Institute for Biotechnology and Genetic Engineering (NIBGE), FasialabadFollow

Abstract

Preeclampsia (PE) is a very common critical condition during pregnancy. As PE is a high-risk condition during pregnancy, occurring in 25% of all pregnancies, worldwide. In women with PE there is an increase in hypertension and albuminuria. Elevated blood pressure can be life-threatening after 20^th week of pregnancy. Single nucleotide variation in gene sequence can be disease causing, among these pathogenic SNPs, a variant in TNF-α, G308A is analyzed in many studies as a causative variant to cause preeclampsia. In this case control study fifty patients and fifty healthy individuals were enrolled for analysis of TNF-α promoter region SNP G308A from Jinnah hospital, Lahore, Pakistan. The genotyping of TNF-α (G308A) rs1800629 polymorphisms was performed by PCR-RFLP method. Data analysis was performed by using SNPStats, statistical tool. The mean age of all patients and controls were calculated, 24.4 ± 6.6 and 25.1 ± 5.3 years, respectively. The frequency of G308A polymorphism was more prevalent in the case group, in association with control group (p

DOI

https://doi.org/10.35691/JBM.0202.0154

Recommended Citation

Ziauddin, A., Ahmad, S., Iqbal, A., Khan, A., & Baig, S. M. (2020). Tumor Necrosis Factor Alpha SNP Variant in Promoter Region G308a, cause Preeclampsia during Pregnancy in Pakistani Women, A Case Control Study, Journal of Bioresource Management, 7 (4).
DOI: https://doi.org/10.35691/JBM.0202.0154