A Transient Receptor Potential Channel Expressed in Taste Receptor Cells

Authors

Cristian A. Pérez
Liquan Huang
Minqing Rong
J. Ashot Kozak, Wright State University - Main CampusFollow
Axel K. Preuss
Hailin Zhang
Marianna Max
Robert F. Margolskee

Document Type

Article

Publication Date

11-2002

Abstract

We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as -gustducin, G₁₃, phospholipase C-₂ (PLC-₂) and inositol 1,4,5-trisphosphate receptor type III (IP₃R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.

Repository Citation

Pérez, C. A., Huang, L., Rong, M., Kozak, J. A., Preuss, A. K., Zhang, H., Max, M., & Margolskee, R. F. (2002). A Transient Receptor Potential Channel Expressed in Taste Receptor Cells. Nature Neuroscience, 5 (11), 1169-1176.
https://corescholar.libraries.wright.edu/ncbp/139

DOI

10.1038/nn952