Pressure (≤4 ATA) Increases Membrane Conductance and Firing Rate in the Rat Solitary Complex
Neuronal sensitivity to pressure, barosensitivity, is illustrated by high-pressure nervous syndrome, which manifests as increased central nervous system excitability when heliox or trimix is breathed at >15 atmospheres absolute (ATA). We have tested the hypothesis that smaller levels of pressure (≤4 ATA) also increase neuronal excitability. The effect of hyperbaric helium, which mimics increased hydrostatic pressure, was determined on putative CO2/H+-chemoreceptor neurons in the solitary complex in rat brain stem slices by intracellular recording. Pressure stimulated firing rate in 31% of neurons (barosensitivity) and decreased input resistance. Barosensitivity was retained during synaptic blockade and was unaffected by antioxidants. Barosensitivity was distributed among CO2/H+-chemosensitive and -insensitive neurons; in CO2/H+-chemosensitive neurons, pressure did not significantly reduce neuronal chemosensitivity. We conclude that moderate pressure stimulates certain solitary complex neurons by a mechanism that possibly involves an increased cation conductance, but that does not involve free radicals. Neuronal barosensitivity to ≤4 ATA may represent a physiological adaptive response to increased pressure or a pathophysiological response that is the early manifestation of high-pressure nervous syndrome.
Mulkey, D. K.,
Henderson, R. A.,
Putnam, R. W.,
& Dean, J. B.
(2003). Pressure (≤4 ATA) Increases Membrane Conductance and Firing Rate in the Rat Solitary Complex. Journal of Applied Physiology, 95 (3), 922-930.