Enhanced Capacity of Females for Early Interferon-γ Production by Natural Killer-Like Cells Following Stimulation by Staphylococcal Enterotoxin A
Staphylococcal enterotoxin A (SEA) induced the production of interferon-γ (IFN-γ) by spleen cells from ICR Swiss mice during the first 24 h of culture. Splenocytes from females produced higher levels of IFN-γ than did those from males at 8,12, and 16 h. By 20 h after SEA stimulation, IFN-γ production by spleen cells from males was similar to that of females. The cell types involved in IFN-γ production in this SEA/spleen cell system were analyzed by depletion studies. Removal of Thy-1+ cells by panning prevented production of IFN-γ in the 24 h after SEA stimulation. In vivo depletion of asialo GM1+ (AGM1+) cells prevented production of IFN-γ through 16 h of culture with SEA, but permitted a modest IFN-γ response at 20 h that was similar in magnitude in both sexes. Following removal of L3T4+ and Lyt-2+ cells by panning, IFN-γ production was detected at 12 h after SEA stimulation and maintained through 24 h of culture with cells from females producing higher levels of IFN-γ. These data suggest that male ICR Swiss mice are deficient in the activity of Thy-1+, AGM1+, L3T4−, and Lyt-2− cells in the early (8–16 h) production of IFN-γ following SEA stimulation of spleen cells.
Tokman, M. G.,
McFarland, H. I.,
Ball, E. J.,
& Bigley, N. J.
(1990). Enhanced Capacity of Females for Early Interferon-γ Production by Natural Killer-Like Cells Following Stimulation by Staphylococcal Enterotoxin A. Journal of Interferon Research, 10 (6), 647-658.