Acute Hypoxia Does Not Influence Intracellular pH in Isolated Rat Carotid Body Type I Cells

Authors

Ryan L. Shapiro
Barbara L. Barr, Wright State University - Main CampusFollow
Robert W. Putnam, Wright State University - Main CampusFollow
Christopher N. Wyatt, Wright State University - Main CampusFollow

Document Type

Conference Proceeding

Publication Date

2012

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Abstract

In order to interpret data obtained from isolated Type I cells during hypoxia it is critical to know whether parameters such as intracellular pH (pH_i) vary during this challenge. Multiple studies have attempted to address this issue but data are contradictory and methods poorly defined (Wilding et al. 1993; Pang and Eyzaguirre 1993). Wilding et al. used BCECF loaded, isolated Type I cells and demonstrated that ‘hypoxia’ did not cause a change in pH_i. However, solutions in these experiments were gassed with 2% oxygen and recording chamber PO₂ was not reported. Conversely, Pang and Eyzaguirre used pH-sensitive microelectrodes and demonstrated that single isolated Type I cells did acidify during a hypoxic challenge whereas clusters of Type I cells did not.

Comments

Presented at the 18th Meeting of the International Society for Arterial Chemoreception (ISAC), Hamilton, Ontario, Canada.

Repository Citation

Shapiro, R. L., Barr, B. L., Putnam, R. W., & Wyatt, C. N. (2012). Acute Hypoxia Does Not Influence Intracellular pH in Isolated Rat Carotid Body Type I Cells. Arterial Chemoreception: From Molecules to Systems, 758, 105-107.
https://corescholar.libraries.wright.edu/ncbp/837

DOI

10.1007/978-94-007-4584-1_14