DNA Ploidy and Proliferation Index of Soft Tissue Sarcomas Determined by Image Cytometry of Fresh Frozen Tissue
The DNA content of Feulgen-stained monolayer imprint preparations from 30 fresh frozen soft tissue and bone sarcomas was analyzed using image cytometry. DNA aneuploidy showed a significant association with increasing tumor grade. Of the grade III tumors, 83.3% (15 of 18) were aneuploid; of the grade I and II sarcomas, 33.3% (4 of 12) were aneuploid (P less than 0.00861). The proliferation index (PI) was determined by the percentage of tumor nuclear area staining with the monoclonal antibody Ki-67. The PI was significantly associated with ploidy and tumor grade. Ki-67 PI was elevated (greater than 2.0%) in 73.7% (14 of 19) of the aneuploid tumors, compared with 30% (3 of 10) of the euploid tumors (P less than 0.04597). Ki-67 PI was greater than 2.0% in 77.8% (14 of 18) of the grade III tumors, compared with 27.2% (3 of 11) of the grade I and II tumors (P less than 0.01773). These findings suggest that DNA ploidy and Ki-67 PI as determined by image analysis may be a useful supplement to tumor grading. The literature examining previous studies of sarcoma DNA ploidy is reviewed.
Herzberg, A. J.,
Kerns, B. M.,
Honkanen, F. A.,
Pence, J. C.,
Iglehart, J. D.,
& Kinney, R.
(1992). DNA Ploidy and Proliferation Index of Soft Tissue Sarcomas Determined by Image Cytometry of Fresh Frozen Tissue. American Journal of Clinical Pathology, 97 (5), S29-S37.