Document Type

Poster

Publication Date

5-3-2021

Identifier/URL

39891024 (Pure)

Abstract

Cytokines play a pivotal role in regulating inflammation, which is a condition that makes thetissue vulnerable to different pathological and physiological conditions. Thus, how cytokinesare regulated is an important area of study. Skin that receives ultraviolet B radiation (UVB),a major pro-oxidative stressor, results in the release of multiple cytokines and chemokineslike tumor necrosis factor (TNF)-alpha and interleukin (IL)-8. Previous studies from our groupand others have demonstrated synergistic release of TNF-alpha when UVB is combined withIL-1 or the lipid mediator Platelet-activating factor (PAF). Of interest, subcellularmicrovesicle particles (MVP) have been proposed to play an important role in intercellularcommunication. Moreover, UVB and PAF agonists cause MVP release in keratinocytes.Therefore, we believe that understanding the role of MVP in these inflammatory responsescould be insightful for photosensitivity mechanisms and to suppress inflammation. Thecurrent study focuses on the combination of low concentrations of PAF agonist and UVB in-vitro and ex-vivo to observe potential synergism in the release of cytokines and MVP. Wealso studied the effects of acid sphingomyelinase (aSMase) inhibitor imipramine, for itsability to modulate both MVP and cytokine release. The application of aSMase inhibitorinhibited the synergistic response of MVP and cytokines allows us to conclude the potentialinvolvement of MVP in the exaggerated response of cytokines from combining UVB andPAF. These studies have potential relevance in understanding abnormal skin reactions suchas photosensitivity.

Download


Included in

Dermatology Commons

Share

COinS