Publication Date


Document Type


Committee Members

Larry G. Arlian (Advisor), David L. Goldstein (Other), Barbara Hull (Committee Member), John O. Stireman (Committee Member), Joseph F. Thomas, Jr. (Other)

Degree Name

Master of Science (MS)


Dermatophagoides pteronyssinus and Dermatophagoides farinae are commercially cultured to produce materials to make extracts for diagnostic tests and immunotherapy. There is some speculation as to how the culture conditions such as time of culture, temperature, relative humidity and food might influence the mite populations and subsequent production of allergens. The purpose of our investigation was to see how temperature influenced the population growth and allergen production of cultured house dust mites. D. pteronyssinus and D. farinae were cultured at 20°C and 25°C. Samples were collected at two week intervals for live mite count, life stage composition and to determine Group 1 and Group 2 allergen concentrations. We saw that temperature did have an influence and that both species showed higher population growth rates and produced higher concentrations of Group 1 and Group 2 allergens than at 25°C compared to 20°C.

Page Count


Department or Program

Department of Biological Sciences

Year Degree Awarded


Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

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Biology Commons