Publication Date

2020

Document Type

Dissertation

Committee Members

Yanfang Chen, M.D., Ph.D. (Advisor); Ji C. Bihl, M.D., Ph.D. (Committee Member); Ravi P. Sahu, Ph.D. (Committee Member)

Degree Name

Doctor of Philosophy (PhD)

Abstract

In this study, we tested the protective effect of UV-B treated HG-HaCaT-Exos on Schwann cells (SCs) injured by HG (high glucose) model. At first, we determined the dose of UV-B radiation and the time for the release of HaCaT-Exos. Furthermore, the Schwann cells were injured through the HG model, isolated by ultracentrifugation from the HaCaT cell culture supernatant were co-incubated within the injured cells. It was found that UV-B treated HaCaT-Exos decrease apoptosis and increase the viability of injured SC by transferring miR-222 from HaCaT cells to them via exosomes, hence protecting them.

Page Count

64

Year Degree Awarded

2020


Included in

Engineering Commons

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