Ji Chen Bihl, M.D., Ph.D. (Advisor); Yanfang Chen, M.D., Ph.D. (Committee Member); Ravi Sahu, Ph.D. (Committee Member)
Master of Science (MS)
Endothelial progenitor cells (EPCs) have been shown to provide beneficial effects on oxidative stress. Exosomes (EXs) released from these stem cells could be one of the major contributors, as they are known to convey the benefit of one cell to another cell via microRNAs (miRNA). At first, we determined that EPCs release more EXs when they are serum-starved for 48 hours., and by determining the microRNA-210 (miR-210) levels in the EXs, we found that miRNA is being transferred from cells to EXs. Meanwhile, miR-210 is gaining popularity in reducing elevated oxidative stress levels. In this study, we investigated the role of endothelial progenitor cells-EXs (EPC-EXs) and the beneficial effects of loading miR-210 into EPC-EXs (miR-210-EPC-EXs) on hypoxia and reoxygenation (H/R) induced reactive oxygen species (ROS) overproduction, apoptosis, and reduced viability of neuronal cells. It was found that EXs were uptaken by neurons and elevated the miR-210 levels in the neurons. In comparison with the vehicle, the EPC-EXs and EXs from scramble transfected EPCs (Scramble-EPC-EXs), were efficient in attenuating neuronal apoptosis, elevated oxidative stress, and restoring cell viability. Whereas the miR-210-EPC-EXs were more efficient in attenuating these inimical effects induced by H/R injury in neurons
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
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