Publication Date
2021
Document Type
Thesis
Committee Members
Sean Harshman, Ph.D. (Committee Co-Chair); Nicholas V. Reo, Ph.D. (Committee Co-Chair); Michael P. Markey, Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Discovery studies have identified many metabolites contained in human sweat. However, quantitative analysis of the sweat metabolome content remains mostly unknown. Furthermore several attributes, including rate, have been defined to affect sweat metabolite content, while other effectors, like diet, remain unknown. This study works to quantitatively define the metabolite impact caused by nutritional supplementation. To better understand the effect diet plays, a LC-MS method was developed focusing on improving resolution and peak width. While the literature provided examples of how diet affected sweat metabolite concentrations, the long-term effects of diet have not been explored. The experiment described here attempts to fill that gap. Partial data separation was found among groups ingesting high and low nutritional supplementation. Several subjects given the high nutritional supplementation had decreased sweat metabolite concentrations after twelve weeks. These results suggest nutritional supplementation can impact the sweat metabolome, and diet should be considered in biomarker discovery experimentation.
Page Count
111
Department or Program
Department of Biochemistry and Molecular Biology
Year Degree Awarded
2021
Copyright
Copyright 2021, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
ORCID ID
0000-0002-4710-7353
