Nancy J. Bigley, Ph.D. (Committee Chair); Dawn P. Wooley, Ph.D. (Committee Member); Marjorie M. Markopoulos, Ph.D. (Committee Member)
Master of Science (MS)
Cardiomyocytes and macrophages have been found to interact via connexin-43 hemichannels. The role of connexin-43, however, is not fully understood. This study shows that these interactions aid in increasing the membrane potential of cardiomyocytes allowing contraction of the cells. HL-1 cardiomyocytes and RAW 264.7 macrophages in coculture increased expression of connexin-43 compared to cardiomyocytes alone. Co-cultures also increased the fluorescence of Di-8-ANEPPS potentiometric dye indicating an increase in cardiomyocyte membrane potential. Treatment with IL-10 and TGF-beta further increased connexin-43 expression and membrane potential. Treatment with SOCS3 inhibited the effects of TGF-beta and IL-10 while having no effect on its own. Confocal imaging can provide visual evidence of these interactions with image layering, 3D imaging and enhanced color.
Department or Program
Microbiology and Immunology
Year Degree Awarded
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