Publication Date


Document Type


Committee Members

Ioana E. Pavel, Ph.D. (Advisor); Travis B. Clark, Ph.D. (Committee Member); Steven R. Higgins, Ph.D. (Committee Member); David A. Dolson, Ph.D. (Committee Member)

Degree Name

Master of Science (MS)


In this study, novel silver nanoparticles (AgNPs) were successfully synthesized and functionalized with an antibacterial agent, namely chlorothymol (denoted C@AgNPs). The resulting colloid (C@AgNPs) was purified by two comparative methods: ultrafiltration and ultracentrifugation. Ultrafiltration proved to be more efficient in purifying and size selecting (10 kD filter) and concentrating the C@AgNPs than ultracentrifugation. The physicochemical properties of the filtered C@AgNPs were then characterized by UV-Vis absorption spectroscopy, inductively coupled plasma optical emission spectroscopy (ICP-OES), Raman spectroscopy, Cytoviva hyperspectral imaging, and Scanning electron microscopy. These measurements confirmed the functionalization of the core AgNPs with chlorothymol and suggest the proposed mechanism of C@AgNP formation through a coordinate covalent bond between the oxygen atom of chlorothymol and the Ag atoms at the nano surface.

Page Count


Department or Program

Department of Chemistry

Year Degree Awarded


Included in

Chemistry Commons