Oleg Paliy (Committee Member), Michael L. Raymer (Committee Member), Nicholas V. Reo (Advisor)
Master of Science (MS)
We describe a methodology that combines urinary metabolomics with a tissue-specific stressor administration to enhance assessment of tissue function. Kidney function in rats was mildly compromised with a sub-acute dose of D-serine and stressed with furosemide. NMR-based metabolomics analyses showed no detectable effects due to D-serine alone; but furosemide or D-serine + furosemide groups, classified separately from each other, and from control. Furosemide alone caused a ca. 2-fold increase in glucose, lactate, choline, and a 30% decrease in TCA intermediates (p≤0.05). D-serine suppressed these effects and produced a 1.7-fold increase in a p-phenolic acid-derivative of tyrosine (PAdY) relative to control (p≤0.05). The PAdY/tyrosine ratio increased 2-fold relative to rats given furosemide alone. D-serine effects were only detectable in furosemide-challenged rats, suggesting that minor disruption in kidney function, induced by low-level D-serine, is manifested by this functional metabolomics methodology. This technique may improve sensitivity for assessment of tissue function and disease
Department or Program
Department of Biochemistry and Molecular Biology
Year Degree Awarded
Copyright 2011, all rights reserved. This open access ETD is published by Wright State University and OhioLINK.