Tolfenamic Acid Induces Cell Apoptosis and Inhibits Collagen Accumulation in Keloid Fibroblasts
Yanfang Chen (Committee Co-chair), David Cool (Committee Member), Richard Simman (Committee Chair)
Master of Science (MS)
Keloid scar is a fibroproliferative disorder which results from the accumulation of collagen through the activation of TGF-B/Smad signaling. Tolfenamic acid (TA) has been found to inhibit collagen synthesis in animal and induce cancer cell apoptosis. In this study, we investigated the effect of DMSO on cell apoptosis, TA on collagen deposition, and TA on collagen type I and smad2 protein expression in human normal fibroblasts (NFs) and human keloid fibroblasts (KFs). In the dose- and exposure time- response assays, we found that 1% DMSO for 48h treatment did not obviously induce NF and KF apoptosis and that 0.55x10-3 M TA for 48h treatment remarkably decreased NF and KF proliferation. Under these experimental conditions, we further found that TA selectively induced cell apoptosis, strikingly decreased collagen production, and effectively inhibited collagen type I expression in KFs. Our data suggested that TA induces cell apoptosis and inhibits collagen accumulation in KFs.
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
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