David Goldstein (Committee Member), Carissa Krane (Committee Member), Michele Wheatly (Advisor), Michele Wheatly (Committee Chair)
Master of Science (MS)
Aquaporins (AQPs) are responsible for transferring water and other small molecules across the cell membrane. Relatively little research has been conducted on AQPs in crustaceans. To study the AQPs in crayfish, I hypothesis that 1) AQP is expressed in the antennal gland (kidney analogue) of crayfish; 2) crayfish AQP will show greater expression in tissues (gill and antennal gland) associated with volume regulation; 3) AQP mRNA expression in antennal gland and gill will increase in premolt and decrease in postmolt compared with intermolt level. RT-PCR and Real-time PCR assay were applied to test the hypothesis. A partial AQP gene was cloned from the antennal gland. This AQP was broadly expressed in all tissues tested. However, there was no significant difference in AQP mRNA expression in antennal gland and gill at three molting stages. This study constitutes a landmark in understanding of AQP function and evolution in crayfish.
Department or Program
Department of Biological Sciences
Year Degree Awarded
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