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Psychosocial factors have the potential for causing psychological or physical harm, perceived psychological demands, job stress, or work-related musculoskeletal disorders (WMSDs). The purpose of the current study was to study the relationship between psychosocial factors including job demands, job control, managerial and colleague support, relationships at work, role conflict, and organizational change, and psychosocial factors, health and the well-being, and WMSDs among flight attendants. A survey, mainly based on the “HSE Indicator Tool” developed by Health and Safety Executive and the Copenhagen Psychosocial Questionnaire (COPSOQ), was distributed in 2009 to flight attendants in a Taiwanese commercial airline by convenience sampling. A total of 145 flight attendants returned the survey. Data showed that 115 (79.3%) of flight attendants received musculoskeletal injuries while at work. It was found that job demand is significantly related to work-related musculoskeletal disorders.