Plant-associated bacteria are an unexplored group of microorganisms that has enormous potential. These bacteria are the source of finding new antimicrobial substances. The present study aimed to isolate and characterize the epiphytes and endophytes from Osmium basilicum and Trigonella foenum graecum plants and to determine their antimicrobial potential against pathogenic bacteria from Nishtar Hospital Multan, Pakistan. The sum of 18 stems and roots along leaf specimens were assembled as of the plant's Osmium basilicum plus Trigonella foenum graecum as of the distinct locales of the Multan city. Overall 73 bacterial strains were isolated and their colony morphology, gram staining, spore staining, and characterization were done. Further 10 epiphytic strains and 14 endophytic strains were selected based on their antimicrobial potential intended for a thorough examination. The activity of the selected microbial isolates was determined against pathogenic bacteria K.pneumoniae, MRSA, E.coli, S .aureus, S.typhimurium as of Nishtar hospital. Out of all strains, only one epiphytic and two endophytic bacterial isolates (methanolic extracts) (obr, obl3, and tfen2 were isolated from root, leaf, and root of Osmium basilicum and Trigonella foenum graecum correspondingly inhibited altogether gram-positive besides gram-negative pathogenic microbes involved in this investigation. The hunt to find novel compounds from plant bacteria will open new horizons in the industrial and agricultural sectors.

Article History

Received: Jan 27, 2022; Accepted: Oct 18, 2022; Published: June 30, 2023