Impact of Imidacloprid on Forebrain Mitochondrial Enzymatic Activities Ec50, Ic50 and Impacts of Mellissa Extracts

Authors

Sarra Zouaoui, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, AlgeriaFollow
Rachid Rouabhi, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, AlgeriaFollow
Chahinez Taib, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, Algeria
Zina Bouteraa, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, Algeria
Salim Gasmi, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, Algeria
Brahim Benaicha, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, Algeria
Nadjiba Toualbia, Laboratory of Toxicology and Ecosystem Pathologies, Echahid Cheikh Larbi Tebessi University, Tebessa, Algeria

Abstract

Neonicotinoids are new generation of neuroactive pesticides, used against a wide specter of insect species, and many non-target beings are affected with these pesticides’ impacts. Our work was focused on the impact of 5, 10, 15, 20, 25 and 30 mg/kg of Imidacloprid the nicotinergic neuronal pathway inhibitor (N-ACh receptors) on the activities of mitochondrial detoxification enzymes (Catalase, SOD, GST and GPx) and acetylcholine esterase (AchE) in forebrain region of Wistar rats. Direct impact on enzymes activities was measured and the IC50, EC50 with or without Mellissa extracts were investigated. Our results showed that the IC₅₀ of imidacloprid on different enzymes are between [7.68 mg/kg - 10.02 mg/kg] CAT_IC50 = 9.09 mg/kg, SOD_IC50 = 8.45 mg/kg, GST_IC50 = 7.68 mg/kg, GPx_IC50 = 9.41 mg/kg. EC50 of Mellissa extracts were investigated between 100 % inhibition and 0 % of each enzyme, CAT_EC50=34.7 mg/kg, SOD_EC50 = 43.92mg/kg, GST_EC50 = 41.66 mg/kg, GPx_EC50 = 41.26 mg/kg. Acetylcholine amount and Acetylcholine esterase activity were assessed and the IC = 10.02 mg/kg, EC50 = 43.35 mg/kg. This investigation showed that the addition of antioxidant natural product was very benefic and neutralized the toxic effect of this pesticide.

Article History

Received: Mar 02, 2024; Accepted: May 02, 2024; Published: Jun 30, 2024

Recommended Citation

Zouaoui, S., Rouabhi, R., Taib, C., Bouteraa, Z., Gasmi, S., Benaicha, B., & Toualbia, N. (2024). Impact of Imidacloprid on Forebrain Mitochondrial Enzymatic Activities Ec50, Ic50 and Impacts of Mellissa Extracts, Journal of Bioresource Management, 11 (2).