Antibiotic Susceptibility Pattern And Esbl Prevalence In Nosocomial Escherichia Coli From Urinary Tract Infection In Kpk, Peshawar

Authors

Muhammad Fawad, Department of Health and Biological Sciences Abasyn University Peshawar, Khyber Pakhtunkhwa-25130-PakistanFollow
Noha E Abdel-razik, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Gizan, Saudi ArabiaFollow
Zain Alhindi, Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah , Saudi ArabiaFollow
Muhammad Usman Amin, Department of Health and Biological Sciences Abasyn University Peshawar, Khyber Pakhtunkhwa-25130-PakistanFollow
Eman Adnan Abu-Seer, Department of Epidemiology, Faculty of Public Health and Health Informatics Umm Al-Qura University Makkah, Saudi ArabiaFollow
Mashael S.Alfaifi, Department of Epidemiology, Faculty of Public Health and Health Informatics Umm Al-Qura University Makkah, Saudi ArabiaFollow
Murad Ali, Department of Zoology, Hazara University Mansehra PakistanFollow
Farkad Bantun, Department of Microbiology and Parasitology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaFollow
Sheraz Dawar, Sarhad university of science and information technology Peshawar. Allied Health sciences Department Medical Lab technologyFollow
Suleman Khan, Department Allied Health Sciences Medical Lab Technology Sarhad University of Science and Information Technology PeshawarFollow
Saad Alghamdi, Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah , Saudi ArabiaFollow

Abstract

The study aimed to evaluate antibiotic susceptibility patterns and extended-spectrum beta-lactamase prevalence in Escherichia coli isolated from urinary tract infections. Total fifty (50) samples of E. coli were obtained from urinary tract infection specimens in Hayatabad Medical Complex and private clinics in Peshawar, Pakistan. The present study obtained 50 E. coli isolates from 100 UTI specimens. The plates were incubated at 35 to 37°C for 24 to 48 hours. Different biochemical tests were carried out to identify the isolates. Antibiotic susceptibility testing and ESBL screening were done per the CLSI (2023) guidelines. The disc diffusion method was used to determine the antibiotic sensitivity pattern. In the present study, the highest ratio was 64 % (n=32), isolates showed resistance to 10 or more antibiotics, indicating them to be multi-drug resistant (MDR) isolates. Among the isolated E. coli strains, 64 % (n=32) were found to be ESBL producers, while the remaining 20 % (10) were found to be non-producers. Out of these 32 ESBL producer isolates, 09 (28.12 %) were positive on the confirmatory test, while the remaining 23(46 %) isolates were negative on the confirmatory test. Our result showed that (78 %) were sensitive to polymyxin B, while (70 %) were susceptible to Gentamicin and cefoperazone/sulbactam. Total (64 %) of the E. coli strains were ESBL producers. It was found that 32 % of ESBL-positive isolates were resistant to various types of antibiotics.

Article History

Received: Jun 10, 2024; Accepted: Aug 15, 2024; Published: Sep 30, 2024

Recommended Citation

Fawad, M., Abdel-razik, N., Alhindi, Z., Usman Amin, M., Abu-Seer, E., S.Alfaifi, M., Ali, M., Bantun, F., Dawar, S., Khan, S., & Alghamdi, S. (2024). Antibiotic Susceptibility Pattern And Esbl Prevalence In Nosocomial Escherichia Coli From Urinary Tract Infection In Kpk, Peshawar, Journal of Bioresource Management, 11 (3).