Dietary Selenium Supplementation Modulates Proliferative Gene Regulators Through Attenuating Cell Cycle Inhibition on Late G1/S Transition in Pre-Pubertal Goat Testis

Authors

Muhammad Awais Soomro, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Moolchand Malhi, Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.Follow
Tarique Ahmed Khokhar, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Faiz Muhammad Khand, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Zafar Ali Khoso, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Maria Sardar, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Muhammad Mohsen Rahimoon, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Awais Bin Shahid, Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.Follow
Muhammad Anees Memon, Shaheed Benazir Bhutto University of Veterinary and Animal Science, 67210 Sakrand, Pakistan.Follow
Nabi Bux Solangi, Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.Follow
Jawed Ali Dhiloo, Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060 Tandojam, Pakistan.Follow

Abstract

Selenium (Se) is essential for normal gonadal improvement in prepubertal male goat. Thus hypothesized that dietary Se may influence late G1/S through M-phase gene networks in the testis, thereby advancing pre-pubertal testicular development via attenuation of inhibitory signals and modulation of late G1 cell-cycle regulators. The total of twenty animals, male goats (3 months old) weighing ~11 kg were arbitrarily given to a basal diet (Control; C, n = 10) or the same diet plus Se-yeast (SY; 0.3 mg/kg; n = 10) for 10 weeks. Basal Se concentration in control diet was 0.037 mg/kg (ICP‑OES Optima 2100‑DV, PerkinElmer). Transcript analysis indicated increases in cyclin E1, Cyclin dependent kinase-2 CDK‑2, cyclin B1, cyclin A, and Cyclin dependent kinase-1 CDK‑1 by 10.5 %, 12.6 %, 11.6 %, 24 %, and 24 %, respectively (P > 0.05), while the CDK inhibitor p‑21 was significantly reduced by 42 % (P < 0.05) in SY vs. C. Because controlled proliferation underpins tissue growth and homeostasis, the marked reduction of p‑21 suggests a mechanism through which Se facilitates regulated proliferation without promoting dysplasia. Collectively, organic Se supplementation appears to foster late G1-phase proliferative signaling by relieving cell-cycle inhibition, thereby supporting testicular development in pre-pubertal goats. The data suggest that Se supplementation level modulates proliferative gene expression, thereby attenuating cell cycle inhibition in pre-pubertal testes of male goat.

Article History

Received: Sep 25, 2025; Accepted: Mar 11, 2026; Published: Mar 30, 2026

Recommended Citation

Soomro, M. A., Malhi, M., Khokhar, T. A., Khand, F. M., Khoso, Z. A., Sardar, M., Rahimoon, M. M., Shahid, A., Memon, M., Solangi, N., & Dhiloo, J. (2026). Dietary Selenium Supplementation Modulates Proliferative Gene Regulators Through Attenuating Cell Cycle Inhibition on Late G1/S Transition in Pre-Pubertal Goat Testis, Journal of Bioresource Management, 13 (1).