Identification and Phylogenetic Analysis of Channa Species from Riverine System of Pakistan Using COI Gene as a DNA Barcoding Marker

Authors

Muhammad Kamran, Department of Zoology, Quaid i Azam University IslamabadFollow
Atif Yaqub, Department of Zoology, Government College University, Lahore, PakistanFollow
Naila Malkani, Department of Zoology, Government College University, Lahore, PakistanFollow
Khalid Mahmood Anjum, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, PakistanFollow
Muhammad Nabeel Awan, Department of Zoology, Government College University, Lahore, PakistanFollow
Hamed Paknejad, Department of Fisheries & Environment Science, University of Agricultural Sciences and Natural Resources Gorgan, IranFollow

Abstract

Channa are the freshwater and important food fish species in Pakistan belonging to family Channidae. However, identification and phylogenetic analysis based on molecular tools of these species in Pakistan was not well known. Herein, the current investigation was conceptualized, which dealt with mitochondrial DNA sequences from three geographically distinct populations of this species from Pakistan’s water system. DNA from fin tissues was extracted. COI region of mtDNA was amplified using universal primers for fish. PCR products were sequenced. Phylogenetic analysis conducted in the present study, i.e. neighbor-joining (NJ) cladogram, maximum likelihood, K2P genetic divergence and histogram suggests that the studied species of family Channidae are genetically different. The K2P intraspecific divergences were lower than interspecific divergences. The clades in the evolutionary tree for three species were clearly separated.

DOI

https://doi.org/10.35691/JBM.0202.0135

Recommended Citation

Kamran, M., Yaqub, A., Malkani, N., Anjum, K. M., Awan, M. N., & Paknejad, H. (2020). Identification and Phylogenetic Analysis of Channa Species from Riverine System of Pakistan Using COI Gene as a DNA Barcoding Marker, Journal of Bioresource Management, 7 (2).
DOI: https://doi.org/10.35691/JBM.0202.0135