Lysosomes Co-Localize with Ryanodine Receptor Subtype 3 to Form a Trigger Zone for Calcium Signalling by NAADP in Rat Pulmonary Arterial Smooth Muscle

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In arterial myocytes the Ca2+ mobilizing messenger NAADP evokes spatially restricted Ca2+ bursts from a lysosome-related store that are subsequently amplified into global Ca2+ waves by Ca2+-induced Ca2+-release from the sarcoplasmic reticulum (SR) via ryanodine receptors (RyRs). Lysosomes facilitate this process by forming clusters that co-localize with a subpopulation of RyRs on the SR. We determine here whether RyR subtypes 1, 2 or 3 selectively co-localize with lysosomal clusters in pulmonary arterial myocytes using affinity purified specific antibodies. The density of: (1) αlgP120 labelling, a lysosome-specific protein, in the perinuclear region of the cell (within 1.5 μm of the nucleus) was ∼4-fold greater than in the sub-plasmalemmal (within 1.5 μm of the plasma membrane) and ∼2-fold greater than in the extra-perinuclear (remainder) regions; (2) RyR3 labelling within the perinuclear region was ∼4- and ∼14-fold greater than that in the extra-perinuclear and sub-plasmalemmal regions, and ∼2-fold greater than that for either RyR1 or RyR2; (3) despite there being no difference in the overall densities of fluorescent labelling of lysosomes and RyR subtypes between cells, co-localization with αlgp120 labelling within the perinuclear region was ∼2-fold greater for RyR3 than for RyR2 or RyR1; (4) co-localization between αlgp120 and each RyR subtype declined markedly outside the perinuclear region. Furthermore, selective block of RyR3 and RyR1 with dantrolene (30 μM) abolished global Ca2+ waves but not Ca2+ bursts in response to intracellular dialysis of NAADP (10 nM). We conclude that a subpopulation of lysosomes cluster in the perinuclear region of the cell and form junctions with SR containing a high density of RyR3 to comprise a trigger zone for Ca2+signalling by NAADP.



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