MTS-Conjugated-Antiactive Caspase 3 Antibodies Inhibit Actinomycin D-Induced Apoptosis
Caspase 3 is critically involved in the pathway of apoptosis. We have conjugated a MTS-transport-peptide to monoclonal and polyclonal anti-caspase-3 antibodies to suppress Actinomycin D-induced apoptosis in human lymphoma T cells. The advantage of using trans-membrane antibodies compared to conventional apoptosis inhibitors is their specific target recognition in the living cell and their lower toxicity compared to conventional apoptosis inhibitors. We could show that a MTS-transport-peptide modified monoclonal anti-caspase-3 antibody reduces Actinomycin D induced apoptosis, as shown by DNA ladder electrophoresis and cell death ELISA. These results indicate that antibodies have a therapeutic potential to inhibit apoptosis in a variety of diseases.
Brown, T. L.,
& Müller, S.
(2003). MTS-Conjugated-Antiactive Caspase 3 Antibodies Inhibit Actinomycin D-Induced Apoptosis. Apoptosis, 8 (6), 631-637.