Recovery by Push-Pull Perfusion of Neurochemicals Released Within the Cuneate Nucleus of the Cat by Somatosensory Stimulation

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The present work describes a combination of techniques for the identification of neurochemicals released within the cuneate nucleus. During electrical stimulation of the superficial radial nerve, the extracellular fluid of the nucleus is continuously sampled by push-pull perfusion. In addition, the population electrical activity of peripheral nerve as well as the activity of cuneate neurons are recorded. Subsequently, the neurochemical content of the sampled fluid is assessed by HPLC analysis. The comparison of sampled fluid content during control (no stimulation) versus stimulation runs indicates that somatosensory stimulation elicits the release of specific neurochemicals within the cuneate nucleus. The possible sources of released neurochemicals are discussed.