Biophysical Properties of a Chloride Channel in the Apical Membrane of a Secretory Epithelial Cell
1. Patch clamp studies on colonic tumor cell line T84 show the presence of chloride channels.
2. The channels are activated by forskolin, PGE2, or 8-Br-cAMP.
3. Single channel conductance was ca 40 pS at the reversal potential, increasing to 70 pS at +80mV and decreasing to 25 pS at −80 mV.
4. Relative permeabilities were I > Br > Cl > F.
Halm, D. R.,
Schoumacher, R. A.,
& Frizzell, R. A.
(1988). Biophysical Properties of a Chloride Channel in the Apical Membrane of a Secretory Epithelial Cell. Comparative Biochemistry and Physiology Part A: Physiology, 90 (4), 597-601.