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Na-K-2Cl cotransporter 2A (NKCC2A), also known as the bumetanide-sensitive cotransporter 1 (BSC1), transports Na+, K+ and Cl- with a stoichiometry of 1:1:2. NKCC2A is considered a kidney specific cotransporter. It is abundantly expressed in apical membrane of the tubular cells in the thick ascending limb of the loop of Henle (TALH) and in the macula densa. However, NKCC2 has also been found at low levels in different cells, including insulin-secreting ones. This secondary active transporter uses the energy stored in the electrochemical gradients of Na and K maintained by the Na/K-ATPase located on the basolateral membrane of the TALH. The gene encoding NKCC2 is Slc12a1, solute carrier family 12 member 1 located on chromosome 2. Mice lacking NKCC2A (NKCC2A-KO) exhibit mild kidney dysfunction. In this lab it was noted that NKCC2A-KO mice express atypical muscle movements, slight tremors and subtle muscle dyscontrol, endure exceptionally well in the forced swim test and had changes in neurotransmitter levels. These observations led to the hypothesis that NKCC2A may be also found in the central nervous system (CNS).
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Fahy , K. E., Lucot , J. B., Castrop , H., & Di Fulvio , M. (2015). Expression of the NKCC2A Cotransporter in Mouse Central Nervous System. .