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Modeling individuals’ cognitive control levels in operational situations is a major challenge for safety in aeronautical industry. Standardized experimental tasks - as the Multi-Attribute Task Battery II (MATB-II) - are dedicated to such a challenge that can be faced using psycho-physiological biosignals. These biosignals are known to be sensitive to cognitive workload, performance, and expertise that are intricate features of MATB-II subtasks. Thus, it remained necessary to investigate whether these features could be set to ensure controlled experimental conditions. Two groups (15 experts in time-pressured decision making and 13 novices) completed 3 MATB-II sub-tasks (tracking, monitoring, and resource management tasks). Biosignals accounting for autonomic nervous system activity were measured continuously, as objective markers of cognition. Confrontation between performance data and (objective and subjective) cognitive markers reported contrasting perspectives regarding the exploitation of MATB-II as a pertinent tool to insure controlled experimental conditions in the context of cognitive control characterization.