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Background. Previous studies and accident analyses have shown that pilots can make roll reversal errors when responding to bank angles shown by the artificial horizon in the Primary Flight Display (PFD). In the current study, we tested whether adding stereoscopic depth cues to the artificial horizon may lead to better bank angle representation due to an improved figure-ground separation between the symbols. Method. Stereoscopic depth cues were created by using a half-silvered mirror multi-layer PFD, which presented the horizon symbol on a lower layer and the aircraft symbol on a higher layer. A group of 23 non-pilots and 18 general aviation pilots were shown left or right bank angles on this multi-layer PFD as well as on a normal single-layer PFD, with the task to roll the wings level using a joystick. Results. In the pilot group, a similar amount of roll-reversal errors was made with both displays (median = 3.3%) with no significant difference, p = 0.635. In the non-pilot group, fewer roll-reversal errors were observed with the multi-layer display, but this difference did not reach significance either (median = 3.3% vs. 5.0%, p = 0.182). In both pilots and non-pilots, the reaction time was longer in the multi-layer display, which reached significance in the non-pilots (p = 0.016) but not in pilots (p = 0.215). Participants noticed that the depth was only visible during the start of the session. Conclusions. The results suggest that using stereoscopic depth cues are not a viable manner to enhance the figure-ground relation in the artificial horizon.