Murine Response to DNA Encoding Herpes Simplex Virus Type-1 Glycoprotein D Targeted to the Liver
Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immunization, while gD-1 DNA was detectable through 14 days post-immunization. Decline of gD-1 expression and detectable gD-1 DNA in the liver correlated with influx of T cells, predominantly CD4(+). The ASOR-poly-L-lysine DNA carrier system promotes hepatic expression of gD-1 and may be useful in vaccination against herpes simplex virus type-1.
Rogers, J. V.,
Hull, B. E.,
Fink, P. S.,
Chiou, H. C.,
& Bigley, N. J.
(2000). Murine Response to DNA Encoding Herpes Simplex Virus Type-1 Glycoprotein D Targeted to the Liver. Vaccine, 18 (15), 1522-1530.