Publication Date
2017
Document Type
Thesis
Committee Members
Ji Bihl (Committee Member), David Cool (Committee Member), Jeffrey Travers (Advisor)
Degree Name
Master of Science (MS)
Abstract
Microvesicle particles (MVP) are found to be important for cellular communication because they contain many bioactive proteins, lipids, cytokines, and nucleic acids. We have previously found that ultraviolet B radiation (UVB) and a Platelet-activating factor agonist (CPAF) can stimulate the release of MVP in keratinocytes. We hypothesized that there may also be an increase in MVP released after thermal burn and that could be involved in pathogenesis of the systemic effects found in some patients. In this thesis various keratinocyte cell lines, mice and human ex vivo skin were used as model systems to test our hypotheses. It was determined that thermal burn significantly increases the release of MVP compared to the untreated groups. UVB, CPAF and thermal burn all seemed to involve acid sphingomyelinase (aSMase), but different MAP kinase pathways. There was also a significant decrease in the cytokine concentration inside MVP after thermal burn, suggesting a possible defense mechanism to prevent cytokine storm.
Page Count
151
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
2017
Copyright
Copyright 2017, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
