Publication Date
2017
Document Type
Thesis
Committee Members
Nancy Bigley (Advisor), Barbara Hull (Committee Member), Dawn Wooley (Committee Member)
Degree Name
Master of Science (MS)
Abstract
In these experiments, SIM-A9 microglia were exposed to Staphylococcus aureus cell wall components lipoteichoic acid (LTA) and peptidoglycan (PGN) at a concentration of 5 ug/mL for six, twelve, eighteen, and twenty-four hours and the cytokine expression, cytoskeleton organization, and cell viability of the cells were observed. Following LTA and PGN exposure, TNF-a; secretion increased at each time interval and was highest observed at 24 hours. No significant IL-10 secretion was detected. Over the 24 hour period, cell viability and cytotoxicity of LTA and PGN treatment groups were not significantly different from the control, indicating the observed inflammatory cytokine response was not due to cell death. These data suggest that LTA and PGN play a predominantly inflammatory role in the first twenty-four hours of S. aureus CNS infection.
Page Count
66
Department or Program
Microbiology and Immunology
Year Degree Awarded
2017
Copyright
Copyright 2017, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
