Publication Date

2018

Document Type

Thesis

Committee Members

Debra Mayes, Ph.D. (Advisor); Adrian Corbett, Ph.D. (Committee Member); Nancy Bigley, Ph.D. (Committee Member)

Degree Name

Master of Science (MS)

Abstract

Females are more protected than males from neurodegenerative diseases until they go through menopause when this protection drops dramatically. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to play a role in memory, PKC, ERK, and connexin-43, in regions of the brain associated with memory decline in an attempt to elucidate significant changes that occur post-estrus. Total whole cell lysates were compared to isolated mitochondrial protein because mitochondrial function is known to be altered during aging. As hypothesized, protein concentrations differed depending on age, gender, and brain region. Additionally, many of these changes occurred within mitochondria but not within whole cell lysates indicating that these are epigenetic alterations. These findings accentuate the complexity of aging and provide insight into the gender-specific cellular processes that occur throughout this process.

Page Count

127

Department or Program

Department of Neuroscience, Cell Biology and Physiology

Year Degree Awarded

2018


Included in

Anatomy Commons

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