Rapid Determination of Nucleotide Content and its Application to the Study of Genome Structure
Document Type
Article
Publication Date
10-11-1991
Abstract
We have developed a sensitive, reliable and accurate procedure for estimating the base composition of small samples of DNAs. This method has been applied to the analysis of genomic DNAs from several sources including large regions of human DNA cloned as yeast artificial chromosomes. To determine whether the human genome is compartmentalized into large segments of homogeneous base composition, we examined the GC content of a 1.2 megabase contig spanning the cystic fibrosis gene.
Repository Citation
Krane, D. E.,
Hartl, D. L.,
& Ochman, H.
(1991). Rapid Determination of Nucleotide Content and its Application to the Study of Genome Structure. Nucleic Acids Research, 19 (19), 5181-5185.
https://corescholar.libraries.wright.edu/biology/219
