Osmolyte Contents of Cultured Astrocytes Grown in Hypoosmotic Medium
Document Type
Article
Publication Date
1-6-1999
Abstract
Primary rat cerebral astrocyte cultures were grown for 2 weeks in isoosmotic medium (305 mosmol) and then placed in similar medium with a reduced NaCl concentration. During the first hour of growth in this moderately hypoosmotic medium (240 mosmol), the cells lose 88% of their taurine contents, 62% of their alanine contents, and 54% of their aspartate contents while regaining normal volume. Loss of these amino acids accounts for 43% of observed volume regulation. Contents of these amino acids remain decreased during 24 h of growth in hypoosmotic medium. In contrast, potassium, glutamate, glutamine, and asparagine contents are not changed, relative to cells in isoosmotic medium, at time points between 1 h and 24 h of hypoosmotic exposure. The data suggest astrocytes contribute to net loss of amino acids, but not potassium, from brains exposed to hypoosmotic conditions in situ.
Repository Citation
Olson, J. E.
(1999). Osmolyte Contents of Cultured Astrocytes Grown in Hypoosmotic Medium. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1453 (1), 175-179.
https://corescholar.libraries.wright.edu/emergency_medicine/244
DOI
10.1016/S0925-4439(98)00090-8
