Control of Astrocyte Volume by Intracellular and Extracellular Ca2+
Document Type
Article
Publication Date
1-1-1990
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Abstract
Astrocytes from primary culture were exposed to conditions that affect intracellular and extracellular Ca2+ concentrations. Astrocyte cell volume was increased approximately 16% after a 30 min exposure to isoosmotic phosphate-buffered saline (PBS) containing the Ca2+ buffer EDTA. Cell volume returned to control values within 30 min of resuspension in normal PBS. Cellular calcium content was not affected by these treatments; however, the recovery of normal cell volume following EDTA exposure was inhibited by 0.1–1.0 mM quinine HCl in a dose-dependent fashion suggesting that a potassium channel controlled by the intracellular Ca2+ concentration is important in this volume response. Intracellular accumulation of an exogenous Ca2+ buffer, BAPTA, also produced cell swelling that persisted following resuspension in normal PBS. Lowering the extracellular Ca2+ concentration with EDTA enhanced the swelling of BAPTA-loaded cells. These data suggest that conditions leading to a decrease in free intracellular Ca2+ concentration may influence astrocyte volume by a mechanism similar to that described in other cell types.
Repository Citation
Olson, J. E.,
Fleischhacker, D.,
Murray, W. B.,
& Holtzman, D.
(1990). Control of Astrocyte Volume by Intracellular and Extracellular Ca2+. GLIA, 3 (5), 405-412.
https://corescholar.libraries.wright.edu/emergency_medicine/265
DOI
10.1002/glia.440030512
