Reduction of Dye Coupling in Glial Cultures by Microinjection of Antibodies Against the Liver Gap Junction Polypeptide

Authors

• F. Edward Dudek, Tulane University School of Medicine
• Valentin K. Gribkoff, Tulane University School of Medicine
• James E. Olson, Wright State University - Main CampusFollow
• Elliot L. Hertzberg, Baylor College of Medicine

Document Type

Article

Publication Date

1-26-1988

Abstract

Intracellular injection of antibodies to the 27-kDa liver gap junction polypeptide have been shown previously to uncouple pairs of cultured mammalian hepatocytes, cardiac myocytes, and ganglionic neurons (Proc. Natl. Acad. Sci. U.S.A., 82 (1985) 2412–2416). In confluent primary cultures of astrocytes, similar injections significantly reduced dye coupling for cells closer than 80 μm to the injected glial cell. Western blots identified a 27-kDa protein in extracts of the astrocyte cultures that cross-react with the gap junction-specific antibodies. These results suggest that hhe homologous gap junction polypeptides exist in liver and glial cells.

Repository Citation

Edward Dudek, F., Gribkoff, V. K., Olson, J. E., & Hertzberg, E. L. (1988). Reduction of Dye Coupling in Glial Cultures by Microinjection of Antibodies Against the Liver Gap Junction Polypeptide. Brain Research, 439 (1-2), 275-280.
https://corescholar.libraries.wright.edu/emergency_medicine/267

DOI

10.1016/0006-8993(88)91484-9