Publication Date


Document Type


Committee Members

Adrian Corbett (Committee Co-chair), Robert Fyffe (Advisor), Larry Ream (Committee Co-chair)

Degree Name

Master of Science (MS)


Peripheral nerve injuries (PNI) cause alternations in central synapses leading to loss of function. The C-bouton synapses onto a-motoneurons in the ventral horn, and has a role in regulating motor output. Following tibial nerve ligation, the somatic C-bouton coverage is depleted (Alvarez et al., 2011), however, it is unknown what happens following crush type injuries. PNI causes neuroglia activation and proliferation that contribute to synaptic alterations, a response that has not been well-characterized in the ventral horn, where motoneurons are located. Therefore, I hypothesize that glia activation following peripheral nerve injury correlates to the degree of depletion of synaptic coverage of C-boutons. To test, I performed Immunohistochemical analysis of rat spinal cord to characterize C-bouton coverage, and activation of glia following two types of PNI. Our results indicate less C-bouton depletion following tibial nerve crush than ligation injuries. In addition, we found less glia activation following crush than ligation injuries.

Page Count


Department or Program

Department of Neuroscience, Cell Biology & Physiology

Year Degree Awarded


Included in

Anatomy Commons