Ji Bihl (Committee Member), David Cool (Committee Member), Jeffrey Travers (Advisor)
Master of Science (MS)
Microvesicle particles (MVP) are found to be important for cellular communication because they contain many bioactive proteins, lipids, cytokines, and nucleic acids. We have previously found that ultraviolet B radiation (UVB) and a Platelet-activating factor agonist (CPAF) can stimulate the release of MVP in keratinocytes. We hypothesized that there may also be an increase in MVP released after thermal burn and that could be involved in pathogenesis of the systemic effects found in some patients. In this thesis various keratinocyte cell lines, mice and human ex vivo skin were used as model systems to test our hypotheses. It was determined that thermal burn significantly increases the release of MVP compared to the untreated groups. UVB, CPAF and thermal burn all seemed to involve acid sphingomyelinase (aSMase), but different MAP kinase pathways. There was also a significant decrease in the cytokine concentration inside MVP after thermal burn, suggesting a possible defense mechanism to prevent cytokine storm.
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
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