The Effect of Gram-Positive Staphylococcus aureus Cell Wall Components Lipoteichoic Acid and Peptidoglycan on Cytokine production, Cytoskeletal Arrangement, and Cell Viability on RAW 264.7 Murine Macrophages
Nancy Bigley (Advisor), Barbara Hull (Committee Member), Dawn Wooley (Committee Member)
Master of Science (MS)
In this study, gram positive Staphylococcus aureus cell wall components such as lipoteichoic acid (LTA) and peptidoglycan (PGN) were used to study the potential inflammatory and anti-inflammatory cytokine response, cytoskeletal arrangement and cell viability on RAW264.7 murine macrophages over 24 hours. The effect of S.aureus LTA and PGN (5 µg/mL) on RAW 264.7 macrophages was evaluated every six hours for twenty-four hours. Inflammatory cytokine (TNF-a) production peaked at 6 hours before decreasing over time. Anti-inflammatory cytokine (IL-10) production peaked between 12 and 18 hours. During the first twenty-four hours, cytotoxicity of treated macrophages, as defined as the release of lactate dehydrogenase, did not increase suggesting the drop in inflammatory cytokine production was not due to cell death. As exposure to S.aureus cell wall components increased over twenty-four hours, cells transformed from a circular-profile M1 phenotype to a more elongated M2 phenotype. The results of this study indicate that the inflammatory response to S.aureus peaks early at 6 hours before being modulated by production of anti-inflammatory IL-10 later at 12 to 18 hours.
Department or Program
Microbiology and Immunology
Year Degree Awarded
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