Vitamin D3 and Suppressor of Cytokine Signaling Proteins Reduces Pro-Inflammatory Cytokines in an Alzheimer's Disease Like-Model Consisting of Microglial and Neuronal Co-Cultures
Nancy Bigley (Advisor), Barbara Hull (Committee Member), Shulin Ju (Committee Member)
Master of Science (MS)
This study examined the inflammatory effects of amyloid-beta (A[beta]42) in a microglial-neuronal co-culture system and determined whether 1a, 25-dihydroxyvitamin D3 (1,25-(OH)2D3) along with suppressor of cytokine signaling (SOCS)1 and SOCS 3 mimetics would attenuate the inflammatory response to A[beta]42. This culture system, when seeded with A[beta]42, serves as an in vitro model for Alzheimer's disease (AD). In a neuronal-microglia co-culture, A[beta]42 stimulated microglia to secrete TNF-a, but with the addition of 1,25-(OH)2D3, TNF-a levels dropped by nearly eight-fold and to near zero values in the presence of both 1,25-(OH)2D3 and SOCS1 and SOCS 3 mimetics. The reduction of the inflammatory cytokine TNF-a by both 1,25-(OH)2D3 and SOCS mimetics, suggests that these molecules may be an effective means of treating AD related inflammation, and that 1,25-(OH)2D3 along with SOCS proteins mimetics should be considered for early onset AD.
Department or Program
Microbiology and Immunology
Year Degree Awarded
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