Publication Date
2020
Document Type
Thesis
Committee Members
Eric Fossum, Ph.D. (Advisor); David Dolson, Ph.D. (Committee Member); Ioana Pavel, Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
A novel organic TADF chromophore was synthesized via a 3-step process to emit a blue fluorescent light upon excitation. The chromophore was covalently attached to a 61% isobutyryl amidated poly(ethyleneimine) (PEI-IBAm0.61) non-viral gene vector utilized in gene transfection experiments. The gene vectors are loaded with a green fluorescent protein (GFP) and are monitored via fluorescence microscopy. The addition of the blue chromophore therefore allows for the monitoring of the movements of the PEI-IBAm0.61 through the full delivery mechanism of the GFP inside the cells. This can give vital information about the overall toxicity of these non-viral gene vectors to biological organisms upon release of the GFP by allowing for the observation of the ultimate fate of the polymer in a biological system.
Page Count
75
Department or Program
Department of Chemistry
Year Degree Awarded
2020
Copyright
Copyright 2020, all rights reserved. My ETD will be available under the "Fair Use" terms of copyright law.
