Publication Date

2020

Document Type

Thesis

Committee Members

Eric Fossum, Ph.D. (Advisor); David Dolson, Ph.D. (Committee Member); Ioana Pavel, Ph.D. (Committee Member)

Degree Name

Master of Science (MS)

Abstract

A novel organic TADF chromophore was synthesized via a 3-step process to emit a blue fluorescent light upon excitation. The chromophore was covalently attached to a 61% isobutyryl amidated poly(ethyleneimine) (PEI-IBAm0.61) non-viral gene vector utilized in gene transfection experiments. The gene vectors are loaded with a green fluorescent protein (GFP) and are monitored via fluorescence microscopy. The addition of the blue chromophore therefore allows for the monitoring of the movements of the PEI-IBAm0.61 through the full delivery mechanism of the GFP inside the cells. This can give vital information about the overall toxicity of these non-viral gene vectors to biological organisms upon release of the GFP by allowing for the observation of the ultimate fate of the polymer in a biological system.

Page Count

75

Year Degree Awarded

2020


Included in

Chemistry Commons

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