Publication Date
2020
Document Type
Thesis
Committee Members
David R. Ladle, Ph.D. (Advisor); Patrick M. Sonner, Ph.D. (Committee Member); Mark M. Rich, M.D., Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Proprioception provides unique sensory feedback about the body’s orientation in space and this sensation is essential for creating specific motor functions and coordinated movement. During neurogenesis, unique genetic markers are expressed and classified as early, transient/middle, and late markers depending on their timing of expression. This study focuses on the expression patterns of two late markers: Heg1 and B3galt2. We investigated how expression of these two markers respond to peripheral nerve injury (PNI). We cloned and generated riboprobes to detect expression of B3galt2 and Heg1 in DRGs using in situ hybridization after transection of the sciatic nerve. B3galt2 hybridization in control, sham, and injured DRG revealed in expression in all DRG neurons. Heg1 hybridizations showed positive specific staining on control tissue. Heg1 expression in lumbar DRG was normalized to 7.83 neurons per 50,000 µm2. However, further investigation would need to be conducted to validate expression in injured DRG neurons.
Page Count
53
Department or Program
Department of Neuroscience, Cell Biology, and Physiology
Year Degree Awarded
2020
Copyright
Copyright 2020, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
