Nancy Bigley (Advisor), Barbara Hull (Committee Member), Osvaldo Lopez (Committee Member)
Master of Science (MS)
The hypothesis for this research was: Herpes simplex virus type 1 (HSV-1) infection of murine keratinocyte cell lines (HEL-30 and PAM-212) treated with mitotic inhibitors leads to silencing of virus replication. At 0.1 (Multiplicity of infection) MOI, PAM-212 keratinocytes showed some cell lysis and viral plaques were seen in HEL-30 keratinocyte cultures. PAM-212 keratinocytes, infected at 0.01 MOI, were more susceptible to the lytic effect of HSV-1 than were HEL-30 cells. In this study, 5-fluoro-2'-deoxyuridine (FUDR) treatment of both keratinocyte cell lines permitted an increase survival of HSV-1- infected keratinocytes especially for PAM-212 cell line which was further examined for the presence of sequestered virus after treatment with FUDR and infection with HSV-1. In this post-mitotic state, HSV-1-infected keratinocytes appeared to contain latent virus as suggested by the lack of virus plaques or cytopathic effect (CPE). After infection, these cells were examined for the presence of replicating HSV-1 in Vero cell overlays. Virus plaques were found, suggesting that latency may have been established.
Department or Program
Microbiology and Immunology
Year Degree Awarded
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