Publication Date

2013

Document Type

Thesis

Committee Members

Adrian Corbett (Advisor), David Ladle (Committee Member), Mark Rich (Committee Member)

Degree Name

Master of Science (MS)

Abstract

Ischemic stroke makes up 87% of all hospital-admitted stroke cases annually; the primary treatment for these cases is intravenous administration of tPA within a 3.5 hour window from stroke onset. A long-term delayed ischemic stroke treatment proposed by this study was a combination of the pharmaceuticals Fluoxetine (SSRI), Simvastatin (statin), and ascorbic acid (Vitamin C). 51 adult rat subjects (10-12 months of age; 44 Sprague Dawley, 7 Long Evans) were given a combination of the drugs for 31 days. Drugs were given through voluntary oral administration via sugar cookie-dough balls to reduce inhibition of neurogenesis through stress-related glucocorticoid production. Drug combinations were as follows: FSA - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin and 20 mg/kg ascorbic acid; FS - 5 mg/kg fluoxetine, 0.5 mg/kg simvastatin; and the vehicle control. Endothelin-induced cortical stroke was administered using 2 different set of coordinates relative to bregma: Group 1 - (AP: 0.0 mm, ML: -2.5 mm) and (AP +1.5 mm; ML: -2.5 mm); Group 2 - (AP: 0.0 mm, ML: -2.5mm) and (AP +2.3; ML -2.5 mm). To analyze functional deficit, rats were subject to Montoya Staircase functional test once pre-stroke and twice post-stroke, and the Forelimb Asymmetry functional test once pre-stroke and thrice post-stroke. Results showed that Long Evans rats sustain a significantly larger infarct volume compared to Sprague Dawley rats using Group 2 coordinates; Group 1 cortical injection coordinates produced a larger infarct than Group 2 coordinates in FSA Sprague Dawley rats; drug treatment showed no effect on total infarct volume, however, this may be attributed to use of generic fluoxetine in Group 2 Sprague Dawley rats.

Page Count

70

Department or Program

Department of Neuroscience, Cell Biology & Physiology

Year Degree Awarded

2013


Included in

Anatomy Commons

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