Katherine Excoffon (Committee Member), Barbara E. Hull (Committee Member), Dawn P. Wooley (Committee Chair)
Master of Science (MS)
The development of a suitable experimental cell model to study HIV latency in primary cells could have a massive effect on the current approaches to eradicate virus in latently infected cells. The main proposal of this paper is to develop an in vitro HIV cell model that represents HIV latency in vivo, then to create a more effective viral vector in order to target HIV reservoirs. For this goal, a directed evolution method is suggested to be used in order to mutate the AAV cap gene to generate a recombinant AAV vector that is capable of infecting primary resting CD4+ T cells previously infected with HIV-1 and in a latent stage.
Department or Program
Microbiology and Immunology
Year Degree Awarded
Copyright 2013, all rights reserved. This open access ETD is published by Wright State University and OhioLINK.