Publication Date


Document Type


Committee Members

David Goldstein (Advisor), Lynn Hartzler (Committee Member), Barbara Hull (Committee Member)

Degree Name

Master of Science (MS)


Aquaporin 1 is a cell membrane integral protein, which functions for water transport through the hydrophobic cell membrane to the cytoplasm. HC-1 is an aquaporin 1-like protein expressed in Hyla chrysoscelis and amphibian that survives at sub-freezing temperatures in its natural habitat. The goal of the current study was to investigate the expression of HC-1 during changing physiological conditions. The expression level of HC-1 was determined in skin, muscle, liver, kidney, and intestines of warm, cold, frozen, and dehydrated frogs using Western blot and immunohistofluorescence. Although HC-1 was widely distributed, the expression level varied among tissues and physiological conditions. In addition, HC-1 was present in both glycosylated and deglycosylated forms, the pattern of glycosylation also varied with tissues and physiological conditions. Thus, I conclude that HC-1 is widely expressed in tissues of Hyla chrysoscelis, and that it is regulated in these tissues according to physiological demands.

Page Count


Department or Program

Department of Biological Sciences

Year Degree Awarded


Included in

Biology Commons