Title

Simple Modification of a Protein Database for Mass Spectral Identification of N-linked Glycopeptides

Document Type

Article

Publication Date

2005

Abstract

We describe an algorithm which modifies a protein database such that during a database search deamidation is limited to asparagines strictly contained within the N-glycosylation consensus sequence. The modified database was evaluated using a dataset created from the shotgun proteomic analysis of N-linked glycopeptides from human blood serum. We demonstrate that the application of the modified database eliminates incorrect glycopeptide assignments, reduces the peptide false-discovery rate, and eliminates the need for manual validation of glycopeptide identifications. Copyright © 2005 John Wiley & Sons, Ltd.

DOI

10.1002/rcm.2162

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